Synthesis of luteinizing hormone-releasing hormone containing tritium-labelled pyroglutamic acid

David H. Coy; Esther J. Coy; Tommie W. Redding; A. V. Schally

doi:10.1016/0006-291X(73)91325-9

Synthesis of luteinizing hormone-releasing hormone containing tritium-labelled pyroglutamic acid

David H. Coy, Esther J. Coy, Tommie W. Redding, A. V. Schally

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A method of preparing luteinizing hormone-releasing hormone (LH-RH) pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH₂, by a combination of solid-phase and classical reactions was employed to conveniently synthesize a tritium-labelled hormone by incorporation of 4-[³H]-pyroglutamic acid into position I of the peptide chain. The tritiated LH-RH possessed a specific radioactivity of 18.3 Ci/mmole and a maximal biological potency.

Original language	English (US)
Pages (from-to)	866-871
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	50
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(73)91325-9
State	Published - Feb 5 1973
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Synthesis of luteinizing hormone-releasing hormone containing tritium-labelled pyroglutamic acid",

abstract = "A method of preparing luteinizing hormone-releasing hormone (LH-RH) pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2, by a combination of solid-phase and classical reactions was employed to conveniently synthesize a tritium-labelled hormone by incorporation of 4-[3H]-pyroglutamic acid into position I of the peptide chain. The tritiated LH-RH possessed a specific radioactivity of 18.3 Ci/mmole and a maximal biological potency.",

author = "Coy, {David H.} and Coy, {Esther J.} and Redding, {Tommie W.} and Schally, {A. V.}",

year = "1973",

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T1 - Synthesis of luteinizing hormone-releasing hormone containing tritium-labelled pyroglutamic acid

AU - Coy, David H.

AU - Coy, Esther J.

AU - Redding, Tommie W.

AU - Schally, A. V.

PY - 1973/2/5

Y1 - 1973/2/5

N2 - A method of preparing luteinizing hormone-releasing hormone (LH-RH) pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2, by a combination of solid-phase and classical reactions was employed to conveniently synthesize a tritium-labelled hormone by incorporation of 4-[3H]-pyroglutamic acid into position I of the peptide chain. The tritiated LH-RH possessed a specific radioactivity of 18.3 Ci/mmole and a maximal biological potency.

AB - A method of preparing luteinizing hormone-releasing hormone (LH-RH) pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2, by a combination of solid-phase and classical reactions was employed to conveniently synthesize a tritium-labelled hormone by incorporation of 4-[3H]-pyroglutamic acid into position I of the peptide chain. The tritiated LH-RH possessed a specific radioactivity of 18.3 Ci/mmole and a maximal biological potency.

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U2 - 10.1016/0006-291X(73)91325-9

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AN - SCOPUS:0015914504

VL - 50

SP - 866

EP - 871

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -

Synthesis of luteinizing hormone-releasing hormone containing tritium-labelled pyroglutamic acid

Abstract

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