TY - JOUR
T1 - Genetic diversity of GBV-C/HGV strains among HIV infected-IVDU and blood donors from Buenos Aires, Argentina
AU - Oubiña, J. R.
AU - Mathet, V.
AU - Feld, M.
AU - Della Latta, M. P.
AU - Ferrario, D.
AU - Verdun, R.
AU - Libonatti, O.
AU - Fernández, J.
AU - Carballal, G.
AU - Sánchez, D. O.
AU - Quarleri, J. F.
N1 - Funding Information:
We are indebted to Lidia Espı́nola for her excellent technical assistance. This work was partly supported by grants PIP N° 6554/97 and 0842/98 from the Consejo Nacional de Investigaciones Cientı́ficas y Técnicas [CONICET]; ME009/J from the Universidad de Buenos Aires, and financial support from Instituto de Investigaciones Biotecnológicas de la Universidad de San Martı́n, Universidad del Salvador and Fundación Florencio Fiorini, Argentina.
PY - 1999/12/15
Y1 - 1999/12/15
N2 - GBV-C/HGV RNA was investigated in serum samples from 70 HIV(+) intravenous drug users (IVDU), as well as from 200 blood donors from Buenos Aires, Argentina. Viral RNA was demonstrated in 21 IVDU by reverse transcription-nested PCR of the 5' UTR. c-DNA amplified products were analyzed and their sequences compared with those downloaded from GenBank. A phylogenetic tree based on 171 sequences demonstrated the presence of three major genogroups, including two subgroups, within local samples, i.e. group 1 (n = 1), 2a (n = 11), 2b (n = 4) and 3 (n = 5). These results agreed entirely with those obtained by a novel RFLP (J. Clin. Microbiol. 37, 1340-1347, 1999) of the same 5' UTR amplicons. As expected, GBV-C/HGV RNA prevalence was significantly higher among IVDU than among blood donors (P < 0.0001), although within the latter group an unexpectedly high rate was also detected, since 11 of 200 sera (5.5%) proved positive. These viral isolates were ascribed either to subgroup 2a (n = 5), subgroup 2b (n = 5) or genogroup 3 (n = 1). Briefly, this partial view of GBV-C/HGV molecular epidemiology in Argentina shows: (i) different rates of GBV-C/HGV infection within both IVDU and blood donors; (ii) a high prevalence of viral RNA among blood donors; and (iii) a predominant circulation of genogroup 2, with minor contribution of groups 3 and 1.
AB - GBV-C/HGV RNA was investigated in serum samples from 70 HIV(+) intravenous drug users (IVDU), as well as from 200 blood donors from Buenos Aires, Argentina. Viral RNA was demonstrated in 21 IVDU by reverse transcription-nested PCR of the 5' UTR. c-DNA amplified products were analyzed and their sequences compared with those downloaded from GenBank. A phylogenetic tree based on 171 sequences demonstrated the presence of three major genogroups, including two subgroups, within local samples, i.e. group 1 (n = 1), 2a (n = 11), 2b (n = 4) and 3 (n = 5). These results agreed entirely with those obtained by a novel RFLP (J. Clin. Microbiol. 37, 1340-1347, 1999) of the same 5' UTR amplicons. As expected, GBV-C/HGV RNA prevalence was significantly higher among IVDU than among blood donors (P < 0.0001), although within the latter group an unexpectedly high rate was also detected, since 11 of 200 sera (5.5%) proved positive. These viral isolates were ascribed either to subgroup 2a (n = 5), subgroup 2b (n = 5) or genogroup 3 (n = 1). Briefly, this partial view of GBV-C/HGV molecular epidemiology in Argentina shows: (i) different rates of GBV-C/HGV infection within both IVDU and blood donors; (ii) a high prevalence of viral RNA among blood donors; and (iii) a predominant circulation of genogroup 2, with minor contribution of groups 3 and 1.
KW - GBV-C
KW - HGV
KW - Molecular epidemiology
KW - Viral genogroups
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U2 - 10.1016/S0168-1702(99)00109-4
DO - 10.1016/S0168-1702(99)00109-4
M3 - Article
C2 - 10581385
AN - SCOPUS:0032697886
VL - 65
SP - 121
EP - 129
JO - Virus Research
JF - Virus Research
SN - 0168-1702
IS - 2
ER -
