Evidence for a pro-calcitonin

B. A. Roos, K. Okano, L. J. Deftos

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


The biosynthesis of calcitonin was studied using radioimmunochemical methods and suspensions of calcitonin-producing cells derived from trout ultimobranchial glands. [14C]leucine was incorporated into cell proteins in a linear fashion for up to 36 hrs. Acid-extracted cellular radioactivity could be precipitated by trichloroacetic acid and calcitonin antiserum. Chromatography of the cell extracts revealed two distinct peaks of radio-immunoassayable and immunoprecipitable calcitonin activity. One peak coeluted with radioiodinated calcitonin, the other as a higher molecular weight species. The relative incorporation of [14C]leucine into the higher and lower molecular weight peaks during "pulse-chase" experiments was consistent with a precursor-product relationship between them.

Original languageEnglish (US)
Pages (from-to)1134-1140
Number of pages7
JournalBiochemical and biophysical research communications
Issue number3
StatePublished - Oct 8 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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